Louisiana Marsh Dieback - Iron and Aluminum tolerance limits and effects on salt marsh plants Spartina alterniflora, Juncus roemerianus, and Avicennia germinans Brown Marsh Task 2.1

Metadata:

• Identification_Information
• Data_Quality_Information
• Entity_and_Attribute_Information
• Distribution_Information
• Metadata_Reference_Information

Identification_Information:

Citation:

Citation_Information:

Originator:

Louisiana State University, Wetland and Biogeochemistry Institute and Department of Oceanography and Coastal Studies

Publication_Date: 2005

Title:

Louisiana Marsh Dieback - Iron and Aluminum tolerance limits and effects on salt marsh plants Spartina alterniflora, Juncus roemerianus, and Avicennia germinans Brown Marsh Task 2.1

Geospatial_Data_Presentation_Form: Digital tabular data

Larger_Work_Citation:

Citation_Information:

Originator:

Barataria-Terrebonne National Estuary Program Scientific-Technical Committee

Publication_Date: 20010207

Title:

Requests for submission of Scope of Services for Marsh Dieback and Nutria Control Emergency Response

Geospatial_Data_Presentation_Form: document

Publication_Information:

Publication_Place: Thibodaux, LA

Publisher:

Barataria-Terrebonne National Estuary Program Scientific-Technical Committee

Online_Linkage: <http://www.brownmarsh.net/reports/brown_marsh_rfp.pdf>

Description:

Abstract:

This data set consists of experimentation on three species of salt marsh plants and the effects that soluble iron and aluminum has on the species in increased amounts. During drought situations, the level of soluble iron and aluminum may increase and possible cause certain plant species to dieback. In the Spring of 2000, coastal Louisiana experienced such drought conditions. Greenhouse experiments were conducted on the three species and consisted of three treatments for both iron and aluminum (total of six treatments) to determine if this was a possible cause of the dieback.

Purpose:

To study the effects of increasing soluble iron and aluminum concentrations on plant growth responses as a result of increased ferrous iron and aluminum availability

Time_Period_of_Content:

Time_Period_Information:

Range_of_Dates/Times:

Beginning_Date: 2002

Ending_Date: 2005

Currentness_Reference: observed

Status:

Progress: Complete

Maintenance_and_Update_Frequency: None planned

Spatial_Domain:

Description_of_Geographic_Extent: Coastal Louisiana

Bounding_Coordinates:

West_Bounding_Coordinate: -93.910681

East_Bounding_Coordinate: -88.777417

North_Bounding_Coordinate: 30.509791

South_Bounding_Coordinate: 28.892107

Keywords:

Theme:

Theme_Keyword_Thesaurus: None

Theme_Keyword: tolerance

Theme_Keyword: brown marsh

Theme_Keyword: salt marsh cord-grass

Theme_Keyword: black mangrove

Theme_Keyword: dieback

Theme_Keyword: black needlerush

Theme_Keyword: concentration

Theme_Keyword: saltmarsh

Theme_Keyword: iron

Theme_Keyword: aluminum

Theme_Keyword: soluble concentration

Theme_Keyword: growth response

Place:

Place_Keyword_Thesaurus: None

Place_Keyword: Louisiana

Place_Keyword: Coastal Louisiana

Place_Keyword: LA

Place_Keyword: Southern Louisiana

Place_Keyword: Terrebonne Basin

Place_Keyword: Barataria Basin

Taxonomy:

Keywords/Taxon:

Taxonomic_Keyword_Thesaurus: None

Taxonomic_Keywords: Spartina alterniflora

Taxonomic_Keywords: Avicennia germinans

Taxonomic_Keywords: Juncus roemerianus

Taxonomic_Keywords: species

Taxonomic_Classification:

Taxon_Rank_Name: Kingdom

Taxon_Rank_Value: Plantae

Taxonomic_Classification:

Taxon_Rank_Name: Division

Taxon_Rank_Value: Magnoliophyta

Taxonomic_Classification:

Taxon_Rank_Name: Class

Taxon_Rank_Value: Liliopsida

Taxonomic_Classification:

Taxon_Rank_Name: Subclass

Taxon_Rank_Value: Commelinidae

Taxonomic_Classification:

Taxon_Rank_Name: Order

Taxon_Rank_Value: Cyperales

Taxonomic_Classification:

Taxon_Rank_Name: Family

Taxon_Rank_Value: Poaceae

Taxonomic_Classification:

Taxon_Rank_Name: Genus

Taxon_Rank_Value: Spartina

Taxonomic_Classification:

Taxon_Rank_Name: Species

Taxon_Rank_Value: alterniflora

Applicable_Common_Name: smooth cordgrass

Taxonomic_Classification:

Taxon_Rank_Name: Order

Taxon_Rank_Value: Juncales

Taxonomic_Classification:

Taxon_Rank_Name: Family

Taxon_Rank_Value: Juncaceae

Taxonomic_Classification:

Taxon_Rank_Name: Genus

Taxon_Rank_Value: Juncus

Taxonomic_Classification:

Taxon_Rank_Name: species

Taxon_Rank_Value: roemerianus

Applicable_Common_Name: needlegrass rush

Taxonomic_Classification:

Taxon_Rank_Name: Class

Taxon_Rank_Value: Magnoliopsida

Taxonomic_Classification:

Taxon_Rank_Name: Subclass

Taxon_Rank_Value: Asteridae

Taxonomic_Classification:

Taxon_Rank_Name: Order

Taxon_Rank_Value: Lamiales

Taxonomic_Classification:

Taxon_Rank_Name: Family

Taxon_Rank_Value: Verbenaceae

Taxonomic_Classification:

Taxon_Rank_Name: Genus

Taxon_Rank_Value: Avicennia

Taxonomic_Classification:

Taxon_Rank_Name: Species

Taxon_Rank_Value: germinans

Applicable_Common_Name: black mangrove

Access_Constraints:

Data generated by the Brown Marsh principal investigators will be available only to the project’s other principal investigators, following the established QA/QC review, for a period of two years. After two years from the finalization of the data, it shall be made available to the public. Please contact the principal investigator for exact date of availability. It is strongly recommended that these data be directly acquired from the Brown Marsh Database Information Management System server and not indirectly through other sources which may have changed the data in some way.

Use_Constraints:

Acknowledgement of the principal investigator as a data source would be appreciated in products developed from these data, and such acknowledgement as is standard for citation and legal practices for data source is expected by users of these data. Users should be aware that comparison with other data sets for the same area from other time periods may be inaccurate due to inconsistencies resulting from changes in mapping conventions, methodologies, data collections and computer processes over time. The principal investigator and the distributor shall not be liable for improper or incorrect use of these data, based on the description of appropriate/inappropriate uses described in this metadata document. The principal investigator and distributor make no claims as to the data’s suitability for other purposes. These data are not legal documents and are not to be used as such.

Point_of_Contact:

Contact_Information:

Contact_Organization_Primary:

Contact_Organization:

Louisiana State University, Wetland Biogeochemistry Institute and Department of Oceanography and Coastal Studies

Contact_Person: Irving A. Mendelssohn

Contact_Position: Professor

Contact_Address:

Address_Type: Physical

Address: Louisiana State University

City: Baton Rouge

State_or_Province: LA

Postal_Code: 70803

Country: USA

Contact_Voice_Telephone: 225 578-6425

Contact_Facsimile_Telephone: 225 578-6423

Contact_Electronic_Mail_Address: imendel@lsu.edu

Native_Data_Set_Environment: Microsoft Excel 2000

Analytical_Tool:

Analytical_Tool_Description: SAS Mixed Model: used to conduct statistical analysis.

Tool_Access_Information:

Online_Linkage: <http://www.sas.com>

Tool_Access_Instructions: See website for information

Tool_Contact:

Contact_Information:

Contact_Organization_Primary:

Contact_Organization: SAS

Contact_Address:

Address_Type: Mailing

Address: 100 SAS Campus Drive

City: Cary

State_or_Province: NC

Postal_Code: 27513-2414

Country: USA

Contact_Voice_Telephone: 919 677-8000

Contact_Facsimile_Telephone: 919 677-4444

Data_Quality_Information:

Attribute_Accuracy:

Attribute_Accuracy_Report:

The accuracy of the measured attributes is dependent of the amount of human error, which is unknown. The accuracy of the calculated attributes is dependent on the accuracy of the equations used and the statistical software.

Logical_Consistency_Report:

For each species, 30 pots were selected for the two metal treatments and further divided into three experimental treatments. Nutrient and pH levels were monitored and maintained through the experiment. All plants were allowed an acclimation period to their new potting environments.

Completeness_Report:

There are no known omissions in the data. During the treatments, concentration levels for the metals were staggered or added before the biweekly solution change in order to quantify a response.

Lineage:

Methodology:

Methodology_Type: Lab

Methodology_Identifier:

Methodology_Keyword_Thesaurus: None

Methodology_Keyword: collection

Methodology_Keyword: acclimation

Methodology_Keyword: acid washed

Methodology_Keyword: plastic bin

Methodology_Keyword: azalea pot

Methodology_Keyword: mesh

Methodology_Keyword: sand

Methodology_Keyword: treatments

Methodology_Keyword: Hoagland's solution

Methodology_Keyword: concentration

Methodology_Keyword: pH

Methodology_Keyword: salinity

Methodology_Keyword: transplanting

Methodology_Description:

Preparation and setup - Salt marsh plants, Spartina alterniflora, Avicennia germinans, and Juncus roemerianus were acquired for the experiment and potted into commerical potting soil and allowed to acclimate to the greenhouse and grow. Once the acclimation period was concluded, the roots of the plants were washed of all soil. Any dead material was removed and the plants were divided into approximately equal sized planting units (parcel of roots and stems). All plastic bins, azalea pots, mesh, and sand used in the experiment were acid washed and rinsed prior to transplanting. The parcels were planted into pots with sand and set into the plastic bins with a nutrient solution. The drain holes of the pots were lined with the mesh to retain sand and allow for solution exchange. The plants were then allowed another acclimation period (to the sand); during this period, the plants were flushed daily with a Hoagland's solution. The solution in the bin was changed biweekly along with removing any algae in the bin and outside of the pots. The water level was maintained at 05 to 1cm above the sand surface. The solution salinity was increased during the biweekly solution changes to the eventual experimental concentration of 23ppt. After this second acclimation, 30 pots of each species were selected; one pot of each species was placed into a bin. A solution of half-strength Hoagland's at 23ppt and a pH of 6.5 in 100L containers. Each bin received approximately 20L of this solution during the biweekly solution change until the beginning of the experimental treatments three months later.

Methodology:

Methodology_Type: Lab

Methodology_Identifier:

Methodology_Keyword_Thesaurus: None

Methodology_Keyword: iron treatment

Methodology_Keyword: aluminum treatment

Methodology_Keyword: metal concentration

Methodology_Keyword: leaf elongation

Methodology_Keyword: stem elongation

Methodology_Keyword: stem density

Methodology_Keyword: leaf density

Methodology_Keyword: stand height

Methodology_Keyword: mortality

Methodology_Keyword: biomass

Methodology_Keyword: photosynthetic

Methodology_Description:

Experimentation and data collection - The bins of plants were separated according to the metal experiment (iron or aluminum) and the experimental treatments (5 bins per treatment), control, hold, and ramp. The solution were continually changed at the biweekly period and pH monitored. The iron experiment was initiated at 100uM Fe and 0 uM Al for all treatments (this concentration was maintained for the control for the experiment's duration). At each solution change, 300ml of the new solution was poured through each plant pot three times and the remaining solution was added to the bin to total 20L. For the iron experiment, the concentration for the Hold and Ramp treatments were increased at each solution change until the end concentration level was reached. For the aluminum experiment, the concentration for the Hold and Ramp treatments were also increased at each solution change until it's end concentration level was reached. Plant measurements were taken during and after the experiment and included: leaf/stem elongation, stem/leaf density, stand height, percent of mortality, live biomass, dead biomass, and photosynthetic response.

Methodology:

Methodology_Type: Lab

Methodology_Identifier:

Methodology_Keyword_Thesaurus: None

Methodology_Keyword: SAS

Methodology_Keyword: statistical analysis

Methodology_Keyword: percentage

Methodology_Keyword: comparison

Methodology_Keyword: mean control

Methodology_Keyword: ANOVA

Methodology_Description:

Analysis - The data were converted to percentage of the control for each species in order to standardize the innate differences among the plant species for all variables except for brown (which was converted to difference of control.) Within the Ramp treatment, the evenly distributed concentrations were selected. Comparisons among the plant species within the Ramp and Hold treatments for each experiment were analyzed separately using SAS 8.0 Mixed Model with repeated measures. We analyzed biomass data within the Ramp and Hold treatments separately with an ANOVA. Plants form significant ANOVAs were compared with least squares means post-test comparison.

Process_Step:

Process_Description: See methodology

Process_Date: 2002-2005

Entity_and_Attribute_Information:

Overview_Description:

Entity_and_Attribute_Overview:

All measured and calculated attributes, such as stand heights, biomass, stem/leaf elongation, etc in this subtask were taken for the overall results, the tolerance limits of iron and aluminum in salt marsh plants.

Entity_and_Attribute_Detail_Citation:

Mendelssohn, Irv, Brown Marsh Project: Task II.1 Growth Response of Brown Marsh Species Populations to Stressor Factors. 2005. Louisiana State University.

Distribution_Information:

Distributor:

Contact_Information:

Contact_Organization_Primary:

Contact_Organization: U.S. Geological Survey, National Wetlands Research Center

Contact_Person: Scott Wilson

Contact_Position: Electrical Engineer

Contact_Address:

Address_Type: Mailing and Physical

Address: 700 Cajundome Blvd

City: Lafayette

State_or_Province: LA

Postal_Code: 70506

Country: USA

Contact_Voice_Telephone: 337 266-8664

Contact_Facsimile_Telephone: 337 266-8513

Contact_Electronic_Mail_Address: scott_wilson@usgs.gov

Distribution_Liability:

Although these data have been processed successfully on a computer system at the designated principal investigator’s facility, no warranty expressed or implied is made regarding the accuracy or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. This disclaimer applies both to individual use of the data and aggregate use with other data. It is strongly recommended that these data are directly acquired from the official Brown Marsh Database Information Management System server or from the principal investigator, and not indirectly through other sources which may have changed the data in some way. It is also strongly recommended that careful attention be given to the contents of the metadata file associated with these data. The Brown Marsh committee shall not be held liable for improper or incorrect use of the data described and/or contained herein. So, these data are provided as is and without any express or implied warranties. Also, use of trade names or commercial products in the metadata file is solely for the purpose of providing specific information, and does not imply recommendation or endorsement. Any downloading and use of these data signifies a user’s agreement to comprehension and compliance of the disclaimer. Insure all portions of metadata are read and clearly understood before using these data in order to protect both user and principal investigators interests.

Standard_Order_Process:

Digital_Form:

Digital_Transfer_Information:

Format_Name: Microsoft Excel

Format_Version_Number: 2000+

File_Decompression_Technique: none

Digital_Transfer_Option:

Online_Option:

Computer_Contact_Information:

Network_Address:

Network_Resource_Name: <http://www.brownmarsh.net>

Fees: None, if available online

Custom_Order_Process: None

Metadata_Reference_Information:

Metadata_Date: 20051107

Metadata_Contact:

Contact_Information:

Contact_Organization_Primary:

Contact_Organization: U.S. Geological Survey/NWRC

Contact_Position: Metadata specialist

Contact_Address:

Address_Type: Mailing and Physical

Address: 700 Cajundome Blvd

City: Lafayette

State_or_Province: LA

Postal_Code: 70506

Country: USA

Contact_Voice_Telephone: 337 266-8500

Contact_Facsimile_Telephone: 337 266-8513

Metadata_Standard_Name: Content Standard for Digital Geospatial Metadata

Metadata_Standard_Version: FGDC-STD-001-1998

Metadata_Extensions:

Profile_Name:

Biological Data Profile of Content Standard for Digital Geospatial Metadata

Online_Linkage: <http://www.fgdc.gov/standards/status/sub5_2.html>

Metadata_Access_Constraints: None

Metadata_Use_Constraints:

Acknowledgement of the National Wetlands Research Center as the metadata source would be appreciated. Please cite the original metadata when using portions to create another record for slightly altered data, such as reprojection.